Scientist II (Cell Therapy / Genome Engineering)

Visualisation in South San ...South San Francisco, United Statesfull timeSenior
$65/hrActive

Job description

Our client, a world leader in biotechnology and life sciences, is seeking a "Scientist II (Cell Therapy / Genome Engineering)". Location: South SanFrancisco, CADuration: Long-Term Contract (Possibility Of Further Extension)Pay Rate: $60-$65/hr on W2Company Benefits: Medical, Dental, Vision, Paid Sick Leave, 401KJob SummaryIn this role, you will be supporting genome engineering and synthetic biology initiatives. This role contributes to cell therapy development and disease‑modeling programs. The successful candidate will support the needs of the Advanced Cell Engineering Hub, particularly in the engineering and profiling of cells for cell therapy development and disease modeling.Key ResponsibilitiesEngineer mammalian cells (cancer cell lines, iPSCs, primary cells) using lentiviral/piggyBac systems and CRISPR gene editing Optimize transfection and gene‑editing workflowsTroubleshoot and refine cell engineering protocolsCharacterize engineered cells using molecular and genomic assaysMaintain, monitor, and quality‑check cell lines in cultureRequired QualificationsBS/MS + 5 years industry experience or PhD in Molecular Biology, Cell Biology, Genetics, or related fieldStrong hands‑on mammalian cell culture experiencePreferred QualificationsExperience with primary T cells and iPSCsSkills in: DNA/RNA/protein extraction, molecular cloning, PCR/RT‑PCR/ddPCR, FACS, CRISPR editing, siRNA/shRNA, SDS‑PAGE, immunoblotting, cell‑based assays, NGS, viral vector workFamiliarity with analysis tools such as Benchling, SnapGene, FlowJo, GraphPad PrismIf interested, please share your updated resume at hr@dawarconsulting.com/lakshmi@dawarconsulting.comOur client, a world leader in biotechnology and life sciences, is seeking a "Scientist II (Cell Therapy / Genome Engineering)". Location: South SanFrancisco, CADuration: Long-Term Contract (Possibility Of Further Extension)Pay Rate: $60-$65/hr on W2Company Benefits: Medical, Dental, Vision, Paid Sick Leave, 401KJob SummaryIn this role, you will be supporting genome engineering and synthetic biology initiatives. This role contributes to cell therapy development and disease‑modeling programs. The successful candidate will support the needs of the Advanced Cell Engineering Hub, particularly in the engineering and profiling of cells for cell therapy development and disease modeling.Key ResponsibilitiesEngineer mammalian cells (cancer cell lines, iPSCs, primary cells) using lentiviral/piggyBac systems and CRISPR gene editing Optimize transfection and gene‑editing workflowsTroubleshoot and refine cell engineering protocolsCharacterize engineered cells using molecular and genomic assaysMaintain, monitor, and quality‑check cell lines in cultureRequired QualificationsBS/MS + 5 years industry experience or PhD in Molecular Biology, Cell Biology, Genetics, or related fieldStrong hands‑on mammalian cell culture experiencePreferred QualificationsExperience with primary T cells and iPSCsSkills in: DNA/RNA/protein extraction, molecular cloning, PCR/RT‑PCR/ddPCR, FACS, CRISPR editing, siRNA/shRNA, SDS‑PAGE, immunoblotting, cell‑based assays, NGS, viral vector workFamiliarity with analysis tools such as Benchling, SnapGene, FlowJo, GraphPad PrismIf interested, please share your updated resume at hr@dawarconsulting.com/lakshmi@dawarconsulting.comOur client, a world leader in biotechnology and life sciences, is seeking a "Scientist II (Cell Therapy / Genome Engineering)".Scientist II (Cell Therapy / Genome Engineering)Location: South SanFrancisco, CALocation:Duration: Long-Term Contract (Possibility Of Further Extension)Duration:Pay Rate: $60-$65/hr on W2Pay Rate:Company Benefits: Medical, Dental, Vision, Paid Sick Leave, 401KCompany Benefits:Job SummaryJob SummaryIn this role, you will be supporting genome engineering and synthetic biology initiatives. This role contributes to cell therapy development and disease‑modeling programs. The successful candidate will support the needs of the Advanced Cell Engineering Hub, particularly in the engineering and profiling of cells for cell therapy development and disease modeling.Key ResponsibilitiesKey Responsibilities
  • Engineer mammalian cells (cancer cell lines, iPSCs, primary cells) using lentiviral/piggyBac systems and CRISPR gene editing
Engineer mammalian cells (cancer cell lines, iPSCs, primary cells) using lentiviral/piggyBac systems and CRISPR gene editing
  • Optimize transfection and gene‑editing workflows
Optimize transfection and gene‑editing workflows
  • Troubleshoot and refine cell engineering protocols
Troubleshoot and refine cell engineering protocols
  • Characterize engineered cells using molecular and genomic assays
Characterize engineered cells using molecular and genomic assays
  • Maintain, monitor, and quality‑check cell lines in culture
Maintain, monitor, and quality‑check cell lines in cultureRequired QualificationsRequired Qualifications
  • BS/MS + 5 years industry experience or PhD in Molecular Biology, Cell Biology, Genetics, or related field
BS/MS + 5 years industry experience or PhD in Molecular Biology, Cell Biology, Genetics, or related field
  • Strong hands‑on mammalian cell culture experience
Strong hands‑on mammalian cell culture experiencePreferred QualificationsPreferred Qualifications
  • Experience with primary T cells and iPSCs
Experience with primary T cells and iPSCs
  • Skills in: DNA/RNA/protein extraction, molecular cloning, PCR/RT‑PCR/ddPCR, FACS, CRISPR editing, siRNA/shRNA, SDS‑PAGE, immunoblotting, cell‑based assays, NGS, viral vector work
Skills in: DNA/RNA/protein extraction, molecular cloning, PCR/RT‑PCR/ddPCR, FACS, CRISPR editing, siRNA/shRNA, SDS‑PAGE, immunoblotting, cell‑based assays, NGS, viral vector work
  • Familiarity with analysis tools such as Benchling, SnapGene, FlowJo, GraphPad Prism
Familiarity with analysis tools such as Benchling, SnapGene, FlowJo, GraphPad PrismIf interested, please share your updated resume at hr@dawarconsulting.com/lakshmi@dawarconsulting.comhr@dawarconsulting.comlakshmi@dawarconsulting.com

Skill set

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