Our client, a world leader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA. Job Duration: Long Term Contract (Possibility Of Extension)Pay Rate : $46.43/hr on W2 Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays. Job Responsibilities: · Design and clone expression vectors and IVT donor plasmids to support research initiatives.· Execute DNA and mRNA transfections in mouse and human cell lines.· Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.· Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides Required Qualifications:Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).Desired proficiency in Snapgene, Prism, and MS Office. If interested, please send us your updated resume athr@dawarconsulting.com/akansha@dawarconsulting.comOur client, a world leader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA. Job Duration: Long Term Contract (Possibility Of Extension)Pay Rate : $46.43/hr on W2 Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays. Job Responsibilities: · Design and clone expression vectors and IVT donor plasmids to support research initiatives.· Execute DNA and mRNA transfections in mouse and human cell lines.· Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.· Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides Required Qualifications:Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).Desired proficiency in Snapgene, Prism, and MS Office. If interested, please send us your updated resume athr@dawarconsulting.com/akansha@dawarconsulting.com

Our client, a world leader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA.

Our client, a world leader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA.“Scientist I”San Diego, CA.

Job Duration: Long Term Contract (Possibility Of Extension)

Job Duration: Long Term Contract (Possibility Of Extension)Job Duration: Long Term Contract (Possibility Of Extension)

Pay Rate : $46.43/hr on W2

Pay Rate : $46.43/hr on W2Pay Rate : $46.43/hr on W2

Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K

Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401KCompany Benefits: Medical, Dental, Vision, Paid Sick leave, 401K

The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.

The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.

Job Responsibilities:

Job Responsibilities:Job Responsibilities:

· Design and clone expression vectors and IVT donor plasmids to support research initiatives.

··Design and clone expression vectors and IVT donor plasmids to support research initiatives.

· Execute DNA and mRNA transfections in mouse and human cell lines.

··Execute DNA and mRNA transfections in mouse and human cell lines.

· Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.

··Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.

· Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

··Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

Required Qualifications:

Required Qualifications:Required Qualifications:

• Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.

Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.

• Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.

Molecular Biology:Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.

• Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.

Functional Assays:Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.

• Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).

Cell Culture:Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).

• Desired proficiency in Snapgene, Prism, and MS Office.

Desired proficiency in Snapgene, Prism, and MS Office.

If interested, please send us your updated resume at

If interested, please send us your updated resume at

hr@dawarconsulting.com/akansha@dawarconsulting.com

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